The Texas Department of Licensing and Regulation have a rule which states that each fighter must rest for three days for each round fought.
The fight at the AT&T Stadium lasted the full eight rounds, meaning neither man is allowed to compete for 24 days.
YouTuber-turned-boxer Paul secured a unanimous points win over the 58-year-old in what was an underwhelming affair.
The show sparked outrage among some fight fans – both before and after Tyson struggled to land a glove on Paul over eight two-minute rounds.
The social media star, 27, was in control throughout the fight with Tyson, who is 31 years his senior, and revealed after the fight that he didn’t knock him out by choice.
‘I’m sorry I didn’t knock him out,’ Paul began, with many agreeing that he did the the right thing by not attempting to knock out the 58-year-old.
Paul also admitted to pulling his punches after he saw his legendary opponent weakening during the bout.
‘After I’d seen him tired I didn’t want to put too much hurt on him, but I wanted the fans to get a good experience,’ he continued. ‘But there was a point where my aggression and violence went away when I wasn’t getting hit. I love Mike.’
Paul is said to have made $40million (£32m) from fighting Tyson, while Iron Mike is said to have been paid around $20m (£16m) for the bout in Arlington, Texas.
An average of 60 million households watch the main event live, with as many as 65 million watching at one time, according to MVP.
The win took Paul to a professional boxing record of 11-1 – with the one defeat coming against Tommy Fury in February last year – and it is unclear what will come next for the 27-year-old.
Many were surprised the fight was even sanctioned after it was initially delayed in June when Tyson suffered an ulcer flareup on a cross-country flight.